Composite

Part:BBa_K3252040:Design

Designed by: Alfin Mohammad Abdillah   Group: iGEM19_ITB_Indonesia   (2019-10-21)


Pr-dCas9-Term


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1447
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1447
    Illegal NheI site found at 1206
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1447
    Illegal BamHI site found at 3485
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1447
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1447
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was assembled by ordering custom gene synthetic.


Source

This part sequence was got from this reference.

Bikard, D., Jiang, W., Samai, P, Hochschild, A., Zhang, F., Marraffini, L. A., (2013). Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system. Nucleic Acids Res. 41(15):7429-37

Holowko, M. B., Wang, H., Jayaraman, P., Poh, C. L., (2016). Biosensing Vibrio cholerae with Genetically Engineered Escherichia coli. ACS Synth. Biol. 2016, 5, 1275-1283

References